Human ES/iPS Cell Monitoring Kit for measuring rBC2LCN in culture supernatant ELISA Wako
Product code: 299-78301
Pack Size: 96 Wells
CE Verification: Research Use Only
- List Price
- £1,492.34
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Product Information
Human ES/iPS Cell Monitoring Kit produced by FUJIFILM Wako Chemical Corporation
rBC2LCN lectin exhibits significant affinity to a mucin-type O-glycan sugar chain called H-type3 (Fuc α1-2Gal β1-3GalNAc) displayed on the podocalyxin binds to the surface of human pluripotent stem cells (hPSCs), human ES cells and human iPS cells. Therefore rBC2LCN is reported as a marker of hPSCs.
The glycoprotein recognised by rBC2LCN is released from hPSCs into cell culture supernatant. This kit can quantitatively determine the released glycoprotein by rBC2LCN-antibody sandwich assay and estimate the number of hPSCs. Because cell culture supernatants, but not the cells themselves are used as sample targeted for assay, it is easily capable of monitoring the increase and decrease of hPSCs in a noninvasive manner during continuous cell culture.
· Increase or decrease in the number of undifferentiated cells can be monitored by analysing culture the supernatants of undifferentiated cells that are undergoing differentiation.
· Culture supernatants (50µl) are used for the assay. Cells are not processed for the analysis and can remain in culture.
· As an ELISA-based method is used, multiple samples can be processed using a simple procedure
96 Wells
Store 2-8°C
rBC2LCN lectin exhibits significant affinity to a mucin-type O-glycan sugar chain called H-type3 (Fuc α1-2Gal β1-3GalNAc) displayed on the podocalyxin binds to the surface of human pluripotent stem cells (hPSCs), human ES cells and human iPS cells. Therefore rBC2LCN is reported as a marker of hPSCs.
The glycoprotein recognised by rBC2LCN is released from hPSCs into cell culture supernatant. This kit can quantitatively determine the released glycoprotein by rBC2LCN-antibody sandwich assay and estimate the number of hPSCs. Because cell culture supernatants, but not the cells themselves are used as sample targeted for assay, it is easily capable of monitoring the increase and decrease of hPSCs in a noninvasive manner during continuous cell culture.
· Increase or decrease in the number of undifferentiated cells can be monitored by analysing culture the supernatants of undifferentiated cells that are undergoing differentiation.
· Culture supernatants (50µl) are used for the assay. Cells are not processed for the analysis and can remain in culture.
· As an ELISA-based method is used, multiple samples can be processed using a simple procedure
96 Wells
Store 2-8°C
Specification
- Brand FUJIFILM Wako Chemicals Europe GmbH – Lab Chem
- Sterile
- CE Certified Research Use Only
- Warranty Period Standard Terms Apply
- Is product classified as dangerous goods? No
- Does the product contain latex?
- Measuring Range NULL
- Sensitivity NULL
- Contents rBC2LCN-Coated plate 96 wells (12x8well strips), HRP-Antibody conjugate, Controls, Diluent, Concentrated Wash Buffer, TMB Solutions, Stop Solution and Plate Seals.
- Detected Chemical rBC2LCN
Storage Details
- Pack Description 96 Wells
- Shipping Condition
- Storage Condition
- Pack Length (m) 0.15
- Pack Width (m) 0.15
- Pack Height (m) 0.2
- Pack Weight (kg) 0.00