17-beta-Estradiol ELISA for serum or plasma 20-2000 pg/ml Dialab
Product code: K00194
- List Price
- £239.95
This is not a regular stock item and therefore not subject to our standard delivery schedule. Please contact us on +44 (0) 2380 483000 for advice on expected delivery date.
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Normal levels:
Children <60 pg/ml
Men <40 pg/ml
Women Follicular phase: 30-100 pg/ml
Ovulatory peak: 130-350 pg/ml
Lutenic phase: 50-180 pg/ml
Menopause <60 pg/ml
This assay is intended for the quantitative determination of 17-β-Estradiol in human serum or plasma for diagnostic use. This 96 well ELISA can provide up to 40 patient tests. 17-β-Estradiol in the sample competes with 17-β-Estradiol-HRP for binding onto the limited number of anti-17-β-Estradiol antibodies coated on the microplate. After incubation, the bound/free separation is performed by a simple solid-phase washing step. Then the enzyme HRP in the bound reaction reacts with the substrate to develop a blue colour which changes to yellow on addition of the stop solution. The colour intensity, as determined by reading absorbance at 450nm, is inversely proportional to the concentration of 17-β-Estradiol in the sample.
Kit contents:
Antibody coated Microtiter wells (Anti-17-β-Estradiol IgG): 12x8 well strips
Calibrator set (0, 20, 120, 300, 600, 2000 pg/ml): 6x0.5ml
Control: 1x0.5ml
Enzyme Conjugate (17-β-Estradiol-HRP): 1x22ml
Substrate Solution (H2O2-TMB 0.26g/L): 1x15ml
Stop Solution (Sulphuric Acid 0.15mol/L): 1x15ml
Once open reagents can be kept at 2-8°C for up to 6 months
Sample volume: 25µl human serum or plasma to be run in duplicate
Incubation time: 150 minutes (120 min at 37°C, 30 min at room temperature)
Measurable range: 20-2000 pg/ml
Sensitivity: 8.68 pg/ml
Store at 2-8°C.
Specification
- Brand Dialab
- Sterile
- CE Certified CE IVD
- Warranty Period Standard Terms Apply
- Is product classified as dangerous goods? No
- Does the product contain latex?
- Shelf Life NULL
- Sensitivity 8.68 pg/ml
- Application This assay is intended for the quantitative determination of 17-β-Estradiol in human serum or plasma for diagnostic use.
- Specificity Specificity for Estradiol 100%, Estrone 2%, Estriol 0.39%, Testosterone 0.02%, Cortisol < 0.007%, Progesterone < 0.0003%, DHEA-S < 0.0001%
- Contents Antibody coated Microtiter wells (Anti-17-β-Estradiol IgG): 12x8 well strips,
Calibrator set (0, 20, 120, 300, 600, 2000 pg/ml): 6x0.5ml
Control: 1x0.5ml
Enzyme Conjugate (17-β-Estradiol-HRP): 1x22ml
Substrate Solution (H2O2-TMB 0.26g/L): 1x15ml
Stop Solution (Sulphuric Acid 0.15mol/L): 1x15ml - Time Required Incubation time: 150 minutes (120 min at 37°C, 30 min at room temperature)
Storage Details
- Pack Description 96 Wells
- Shipping Condition
- Storage Condition
- Pack Length (m) 0.12
- Pack Width (m) 0.12
- Pack Height (m) 0.1
- Pack Weight (kg) 0.00