Nucleic Acid Plus BCT™
Nucleic Plus BCT™ is a direct draw whole blood collection tube designed to preserve plasma integrity under real-world collection, shipping and processing conditions for workflows involving cell-free DNA, cell-free RNA, miRNA, extracellular vesicles (EVs), and select plasma protein workflows.
Please note that Nucleic Acid Plus BCT is functionally equivalent to Protein Plus BCT. The updated name more clearly reflects the product’s role within Streck’s multi‑analyte stabilisation portfolio.
Improved workflows
Applications
Accurate analysis of plasma analytes including cell-free DNA, cell-free RNA, extracellular vesicles (EVs), and plasma proteins such as cytokines can be compromised by delayed blood sample processing, handling, and shipping. Deterioration of blood components, including blood cell degradation, ex vivo haemolysis, and platelet activation, may alter plasma analyte concentrations and lead to inconsistent or misleading results.
The preservative reagent contained in Nucleic Acid Plus BCT stabilises nucleated blood cells and erythrocytes while minimising ex vivo haemolysis and platelet activation, thereby limiting the extraneous release of cellular materials such as genomic DNA from white blood cells, EVs, RNA, and proteins from all blood cells, especially immature red blood cells and activated platelets. This stabilisation helps maintain draw-time plasma analyte concentrations during whole blood storage.
Samples collected in Nucleic Acid Plus BCT are stable for up to 7 days for nucleic acid–based analytes and up to 5 days for plasma proteins, allowing convenient sample collection, transport, and storage. Protein stability duration is analyte dependent.
For Research Use Only. Not for use in diagnostic procedures. Nucleic Acid Plus BCT should only be used for research or the development of new assays.
Features
Stabilises cfDNA, cfRNA, EVs and select plasma protein markers.
Limits ex vivo platelet activation and haemolysis.
Compatible with mass spectrometry and immunoassay-based proteomic analysis.
Benefits
Minimises contamination of blood cell-secreted proteins into plasma, providing sample integrity during storage, shipping and handling of blood samples.
Reduces the need for immediate sample processing and analysis.
Room temperature storage reduces costs and complications associated with cold chain shipping.
Allows for the batching of samples, optimising laboratory workflow
95 kPa pressure differential tested for compliance with UN3373-Pl650 and 49 CFR 173.196(a)(6) specimen transport regulations
Product Equivalence
Streck confirms that Nucleic Acid Plus BCT is equivalent to Protein Plus BCT. There are no changes to:
- Formulation
- Tube components
- Manufacturing process
- Performance characteristics
- Intended research applications for same reagent formulation now spans cfNA, miRNA, EV and select plasma proteins
Data generated using Protein Plus BCT remains valid and applicable.
Literature
Publications
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Protein Plus BCT™: A Novel Blood Collection Tube for Stabilizing Plasma Proteins in Ambient Whole Blood Storage
Jing Li, et al. - Streck
09/02/24 -
Combined use of Protein Plus BCT™ and Seer Proteograph™ XT ensures reliable sample integrity for extensive plasma proteome analysis
Rachel M. Miller et al. - Streck
09/02/24
Please note that Nucleic Acid Plus BCT is functionally equivalent to Protein Plus BCT.