Follicle Stimulating Hormone (FSH) ELISA for serum or plasma 1-100 mIU/ml Dialab
Product code: Z05302
- List Price
- £89.00
This is not a regular stock item and therefore not subject to our standard delivery schedule. Please contact us on +44 (0) 2380 483000 for advice on expected delivery date.
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Male: 2.0-14.0 mIU/mL
Female:
Follicular phase: 2.0-10.0 mIU/mL
Ovulatory phase: 6.0-24.0 mIU/mL
Luteal phase: 1.5-8.0 mIU/mL
Post-menopausal: 17.0-95.0 mIU/mL
Pregnant female: 0.0-11.6 mIU/mL
This assay is intended for the quantitative determination of FSH in human serum or plasma for diagnostic use. This 96 well ELISA can provide up to 42 patient tests. FSH in the sample is bound to the antibody that coats the 96 well plate. The antigen-antibody complex is then reacted with anti-FSH horseradish peroxidase (HRP) conjugate resulting in the FSH antigen being sandwiched between the solid phase antibody and the enzyme conjugate. Then the enzyme HRP in the bound reaction reacts with the substrate to develop a blue colour which changes to yellow on addition of the stop solution. The colour intensity, as determined by reading absorbance at 450nm, is proportional to the concentration of FSH in the sample.
Kit contents:
Antibody coated Microtiter wells (Anti-FSH, monoclonal): 12x8 well strips
Calibrator set (0.0 mIU/ml x 1ml; 5, 12, 40, 100 mIU/ml x 0.4ml)
Enzyme Conjugate (Anti-FSH-HRP): 1x11ml
Substrate Solution (H2O2-TMB 0.25g/L): 1x11ml
Stop Solution (Sulphuric Acid 0.15mol/L): 1x11ml
Sample volume: 50µl human serum or plasma to be run in duplicate
Incubation time: 80 minutes (60 min at room temperature, 20 min in the dark at room temperature)
Calibration range: 1-100 mIU/mL
Sensitivity: 1.00 mIU/mL
Specificity: hFSH 100.00%, hLH < 1.0%, hCG < 1.0%, PRL < 1.0%
Store at 2-8°C.
Specification
- Brand Dialab
- Sterile
- CE Certified CE IVD
- Warranty Period Standard Terms Apply
- Is product classified as dangerous goods? No
- Does the product contain latex?
- Shelf Life NULL
- Sensitivity 1.00 mIU/mL
- Application This assay is intended for the quantitative determination of FSH in human serum or plasma for diagnostic use.
- Specificity hFSH 100.00%, hLH < 1.0%, hCG < 1.0%, PRL < 1.0%
- Contents Antibody coated Microtiter wells (Anti-FSH, monoclonal): 12x8 well strips; Calibrator set (0.0 mIU/ml x 1ml; 5, 12, 40, 100 mIU/ml x 0.4ml); Enzyme Conjugate (Anti-FSH-HRP): 1x11ml; Substrate Solution (H2O2-TMB 0.25g/L): 1x11ml
Stop Solution (Sulphuric Acid 0.15mol/L): 1x11ml - Time Required Incubation time:80 minutes (60 min at room temperature, 20 min in the dark at room temperature)
Storage Details
- Pack Description 96 Wells
- Shipping Condition
- Storage Condition
- Pack Length (m) 0.12
- Pack Width (m) 0.12
- Pack Height (m) 0.1
- Pack Weight (kg) 0.00