Androstenedione ELISA for serum or plasma 0.1-10 ng/ml Dialab
Product code: K00197
- List Price
- £233.00
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Female:
Follicular phase: 0.75-3.1 ng/ml
Luteinic phase: 0.94-3.2 ng/ml
Male: 0.60-2.7 ng/ml
This assay is intended for the quantitative determination of androstenedione in human serum or plasma for diagnostic use. This 96 well ELISA can provide up to 40 patient tests. Androstenedione in the sample competes with horseradish peroxidase (HRP) labelled androstenedione for binding onto the limited number of anti-androstenedione coated on the microplate. After incubation, the bound/free separation is performed by a simple- solid-phase washing. The enzyme in the bound-fraction reacts with the substrate to develop a blue colour which changes to yellow on addition of the stop solution. The colour intensity, as determined by reading absorbance at 450nm, is inversely proportional to the concentration of androstenedione in the sample.
Kit contents:
Antibody coated Microtiter wells (Anti-androstenedione IgG): 12x8 well strips
Calibrator set (0.0; 0.1, 0.4, 1.2, 4.0, 10.0 ng/ml x 1.0ml)
Enzyme Conjugate (Anti-Androstenedione-HRP): 1x21ml
Substrate Solution (H2O2-TMB 0.25g/L): 1x15ml
Stop Solution (Sulphuric Acid 0.15mol/L): 1x11ml
Wash Buffer 10X (0.2M Phosphate buffer, pH 7.4, NaCl 160g/L, Tween-20 10g/L) : 1x50ml
Sample volume: 25µl human serum or plasma to be run in duplicate
Incubation time: 75 minutes (60 min at room temperature, 15 min in the dark at room temperature)
Measuring range: 0.1-10.0 ng/mL
Sensitivity: 0.01ng/ml
Specificity: Androstenedione 100%, Testosterone < 0.001%, Epitestosterone 0.04%, 5 α-dihydrotestosterone 0.05%, DHEA 0.05%, Progesterone 0.007%
Store at 2-8°C.
Specification
- Brand Dialab
- Sterile
- CE Certified CE IVD
- Warranty Period Standard Terms Apply
- Is product classified as dangerous goods? No
- Does the product contain latex?
- Shelf Life NULL
- Sensitivity 0.01ng/ml
- Application This assay is intended for the quantitative determination of androstenedione in human serum or plasma for diagnostic use.
- Specificity Androstenedione 100%, Testosterone < 0.001%, Epitestosterone 0.04%, 5 α-dihydrotestosterone 0.05%, DHEA 0.05%, Progesterone 0.007%, Oestrone 0.007%, Cortisol 0.008%, DHEA-S 0.027%, 17βOestradiol < 0.001%, Oestriol < 0.001%, Aldosterone < 0.001%
- Contents Antibody coated Microtiter wells (Anti-androstenedione IgG): 12x8 well strips
Calibrator set (0.0; 0.1, 0.4, 1.2, 4.0, 10.0 ng/ml x 1.0ml)
Enzyme Conjugate (Anti-Androstenedione-HRP): 1x21ml
Substrate Solution (H2O2-TMB 0.25g/L): 1x15ml
Stop Solution (Sulphuric Acid 0.15mol/L): 1x11ml
Wash Buffer 10X (0.2M Phosphate buffer, pH 7.4, NaCl 160g/L, Tween-20 10g/L) : 1x50ml - Time Required Incubation time: 75 minutes (60 min at room temperature, 15 min in the dark at room temperature)
Storage Details
- Pack Description 96 Wells
- Shipping Condition
- Storage Condition
- Pack Length (m) 0.12
- Pack Width (m) 0.12
- Pack Height (m) 0.1
- Pack Weight (kg) 0.00