Dehydroepiandrosterone-Sulphate (DHEA-S) ELISA for serum or plasma 0.1-10.0 µg/ml Dialab
Product code: K00203
- List Price
- £233.00
This is not a regular stock item and therefore not subject to our standard delivery schedule. Please contact us on +44 (0) 2380 483000 for advice on expected delivery date.
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Women µg/ml Men µg/ml
Newborns 0.9-1.8 0.9-1.8
Before puberty 0.25-1.0 0.25-1.0
Adults 0.9-3.6 0.9-3.6
After menopause <0.25-1.0 N/A
Pregnancy 0.25-1.8 N/A
This assay is intended for the quantitative determination of DHEA-S in human serum or plasma for diagnostic use. This 96 well ELISA can provide up to 40 patient tests. DHEA-S in the sample competes with horseradish peroxidase (HRP) labelled DHEA-S for binding onto the limited number of anti-DHEA-S sites coated on the microplate. After incubation, the bound/free separation is performed by a simple- solid-phase washing. The enzyme in the bound-fraction reacts with the substrate to develop a blue colour which changes to yellow on addition of the stop solution. The colour intensity, as determined by reading absorbance at 450nm, is inversely proportional to the concentration of DHEA-S in the sample.
Antibody coated Microtiter wells (Anti-DHEA-S IgG): 12x8 well strips
Calibrator set (0.0, 0.1, 0.4, 1.0, 4.0, 10.0 µg/ml x 1.0ml)
DHEA-S Control: 1x1ml
Sample Diluent 5X (HEPES 187mM, pH 7.5, BSA 0.5g.l): 1x20ml
Enzyme Conjugate (Anti-DHEA-S-HRP): 1x12ml
Substrate Solution (H2O2-TMB 0.26g/L): 1x15ml
Stop Solution (Sulphuric Acid 0.15mol/L): 1x15ml
Sample volume: 30µl human serum or plasma to be run in duplicate
Incubation time: 75 minutes (60 min at room temperature, 15 min in the dark at room temperature)
Measuring range: 0.1-10.0 µg/mL
Sensitivity: 0.03 µg/ml
Specificity: DHEA-S 90%, DHEA 100%, Androsterone-S-NA 48%, Androstenedione 20%, Testosterone 0.01%, Progesterone < 0.01%, Oestrone 0.01%, Cortisol 0.001%. Cholesterol 0.001%
Store at 2-8°C.
Specification
- Brand Dialab
- Sterile
- CE Certified CE IVD
- Warranty Period Standard Terms Apply
- Is product classified as dangerous goods? No
- Does the product contain latex?
- Shelf Life NULL
- Sensitivity 0.03µg/ml
- Application This assay is intended for the quantitative determination of DHEA-S in human serum or plasma for diagnostic use.
- Specificity DHEA-S 90%, DHEA 100%, Androsterone-S-NA 48%, Androstenedione 20%, Testosterone 0.01%, Progesterone < 0.01%, Oestrone 0.01%, Cortisol 0.001%. Cholesterol 0.001
- Contents Antibody coated Microtiter wells (Anti-DHEA-S IgG): 12x8 well strips
Calibrator set (0.0, 0.1, 0.4, 1.0, 4.0, 10.0 µg/ml x 1.0ml)
DHEA-S Control: 1x1ml
Sample Diluent 5X (HEPES 187mM, pH 7.5, BSA 0.5g.l): 1x20ml
Enzyme Conjugate (Anti-DHEA-S-HRP): 1x12ml
Substrate Solution (H2O2-TMB 0.26g/L): 1x15ml
Stop Solution (Sulphuric Acid 0.15mol/L): 1x15ml - Time Required Incubation time: 75 minutes (60 min at room temperature, 15 min in the dark at room temperature)
Storage Details
- Pack Description 96 Wells
- Shipping Condition
- Storage Condition
- Pack Length (m) 0.12
- Pack Width (m) 0.12
- Pack Height (m) 0.1
- Pack Weight (kg) 0.00